Yanying Yu     email
Helmholtz Institute for RNA-based Infection Research

Supervisor:
Jun. Prof. Dr. Lars Barquist (Würzburg)
Promotion Committee:
Jun. Prof. Dr. Lars Barquist (Würzburg)
Prof. Dr. Chase Beise (Würzburg)
Dr. Ana Rita Brochado (Würzburg)
Dr. Tobias Müller (Würzburg)

Development of CRISPR/Cas method for Functional Genomics

Genome-wide screens with clustered regularly inter-spaced short palindromic repeats (CRISPR)/Cas9 system has been proven to be a powerful tool for understanding the complex relationship between genotype and phenotype. To further investigate the potential of different CRISPR/Cas systems, my PhD project aims to develop novel methods based on genome-wide screening with CRISPR/dCas9, CRISPR base editor and CRISPR/Cas13a in E. coli. However, despite the popularity of CRISPR/Cas9 system, the existing library design guidelines are limited based on single guide RNA (sgRNA) in mammalian cells. Compared to CRISPR/Cas9, the other two systems are relatively new and under-investigated, thus the requirements for library design remain more ambiguous. Therefore, firstly I set out to gain better understanding in individual systems to optimize library design and also to explore important features using (automated) machine learning. With optimized libraries, I aim to gain insights in genetic interactions, transcription and translation regulation.