Lukáš Pekárek        email
Helmholtz Institute for RNA-based Infection Research

Supervisor:
Jun. Prof. Dr. Neva Caliskan (Würzburg)
Promotion Committee:
Jun. Prof. Dr. Neva Caliskan (Würzburg)
Prof. Dr. Utz Fischer (Würzburg)
Prof. Dr. Markus Sauer (Würzburg)

Single-molecule approaches to study frameshifting mechanisms and efficiency

Programmed ribosome frameshifting (PRF) events can lead to a controlled change of the reading frame on specific mRNAs. Such non-standard canonical translation events can drive production of alternative peptides from the same mRNA in all domains of life. -1 PRF requires specific cis-acting elements - a slippery sequence of repetitive nucleotides followed by a stable RNA structure. PRF efficiency is also affected by trans-acting factors (TAF) such as proteins, miRNAs and metabolites (1, 2, 3, 4). The interactions of these factors with the RNA and the translation machinery have not yet been completely understood. Recent advances in single-molecule (SM) techniques allow studying non-standard translation events at the molecular level and thus unveil hitherto unrecognised details

(1) Napthine et al (2017) Nat. Commun.
(2) Caliskan et al (2015) Trends Biochem Sci.

(3)
Belew et al (2014) Nature.
(4)
Li et al (2014) PNAS.