Franziska Wimmer     email
Helmholtz Institute for RNA-based Infection Research

Prof. Dr. Chase Beisel (Würzburg)
Promotion Committee:
Prof. Dr. Chase Beisel (Würzburg)
Prof. Dr. Cynthia Sharma (Würzburg)
Jun. Prof. Dr. Neva Caliskan (Würzburg)

Alternative functions of CRISPR-Cas

My project is to investigate alternative functions of CRISPR-Cas systems by studying Xanthomonas albilineans, a bacterial pathogen of sugarcane. This bacterium encodes two CRISPR-Cas systems with the largest reported number of self-targeting CRISPR spacers detected so far, raising the possibility of alternative CRISPR functions for genome-regulating purposes. To investigate the properties of these CRISPR-Cas systems, we will use TX-TL as well as in vivo approaches to assess Cas protein requirements, DNA binding/cleavage properties, and the identification of anti-CRISPR proteins.