Original Publikationen:

Liang C, Rios-Miguel AB, Jarick M, Neurgaonkar P, Girard M, François P, Schrenzel J, Ibrahim ES, Ohlsen K, Dandekar T (2021)
Staphylococcusaureus Transcriptome Data and Metabolic Modelling Investigate the Interplay of Ser/Thr Kinase PknB, Its Phosphatase Stp, the glmR/yvcK Regulon and the cdaA Operon for Metabolic Adaptation
Microorganisms
9(10):2148

Stelzner K, Boyny A, Hertlein T, Sroka A, Moldovan A, Paprotka K, Kessie D, Mehling H, Potempa J, Ohlsen K, Fraunholz MJ, Rudel T (2021)
Intracellular Staphylococcus aureus employs the cysteine protease staphopain A to induce host cell death in epithelial cells
PLoS pathogens 17(9):e1009874

Masota NE, Vogg G, Ohlsen K, Holzgrabe U (2021)
Reproducibility challenges in the search for antibacterial compounds from nature
PLoS One 16(7):e0255437

Mühlberg E, Umstätter F, Domhan C, Hertlein T, Ohlsen K, Krause A, Kleist C, Beijer B, Zimmermann S, Haberkorn U, Mier W, Uhl P (2020)
Vancomycin-Lipopeptide Conjugates with High Antimicrobial Activity on Vancomycin-Resistant Enterococci
Pharmaceuticals (Basel) 29;13(6):110

Saedtler M, Förtig N, Ohlsen K, Faber F, Masota N, Kowalick K, Holzgrabe U, Meinel L (2020)
Antibacterial Anacardic Acid Derivatives
ACS Infect Dis 10;6(7):1674-1685

Umstätter F, Domhan C, Hertlein T, Ohlsen K, Mühlberg E, Kleist C, Zimmermann S, Beijer B, Klika KD, Haberkorn U, Mier W, Uhl P (2020)
Vancomycin Resistance Is Overcome by Conjugation of Polycationic Peptides
Angew Chem Int Ed Engl 2;59(23):8823-8827

Yasrebi K, Schade N, Adeniyi ET, Wecklein B, Ymeraj A, Hertlein T, Ohlsen K, Suzen S, Lalk M, Ströhl D, Hilgeroth A (2020)
Novel effective antibacterial small-molecules against Staphylococcus and Enterococcus strains
Future Med Chem 12(13):1205-1211

Martins Gomes SF, Westermann AJ, Sauerwein T, Hertlein T, Förstner KU, Ohlsen K, Metzger M, Shusta EV, Kim BJ, Appelt-Menzel A, Schubert-Unkmeir A (2019)
Induced Pluripotent Stem Cell-Derived Brain Endothelial Cells as a Cellular Model to Study Neisseria meningitidis Infection
Front Microbiology 29;10:1181

Fan SH, Ebner P, Reichert S, Hertlein T, Zabel S, Lankapalli AK, Nieselt K, Ohlsen K, Götz F (2019)
MpsAB is important for Staphylococcus aureus virulence and growth at atmospheric CO(2) levels
Nature Communication 9;10(1):3627

Nguyen MT, Saising J, Tribelli PM, Nega M, Diene SM, François P, Schrenzel J, Spröer C, Bunk B, Ebner P, Hertlein T, Kumari N, Härtner T, Wistuba D, Voravuthikunchai SP, Mäder U, Ohlsen K, Götz F (2019)
Inactivation of farR Causes High Rhodomyrtone Resistance and Increased Pathogenicity in Staphylococcus aureus
Front Microbiology 2019 28;10:1157

Trübe P, Hertlein T, Mrochen DM, Schulz D, Jorde I, Krause B, Zeun J, Fischer S, Wolf SA, Walther B, Semmler T, Bröker BM, Ulrich RG, Ohlsen K, Holtfreter S (2019)
Bringing together what belongs together: Optimizing murine infection models by using mouse-adapted Staphylococcus aureus strains
Int J Med Microbiol 309(1):26-38

Ashraf K, Yasrebi K, Adeniyi ET, Hertlein T, Ohlsen K, Lalk M, Erdmann F, Hilgeroth A (2019)
Antistaphylococcal evaluation of indole-naphthalene hybrid analogs
Drug Des Devel Therapy 10;13:275-283

Nagel A, Michalik S, Debarbouille M, Hertlein T, Gesell Salazar M, Rath H, Msadek T, Ohlsen K, van Dijl JM, Völker U, Mäder U (2018)
Inhibition of Rho Activity Increases Expression of SaeRS-Dependent Virulence Factor Genes in Staphylococcus aureus, Showing a Link between Transcription Termination, Antibiotic Action, and Virulence
MBio 18;9(5). pii: e01332-18

Jarick M, Bertsche U, Stahl M, Schultz D, Methling K, Lalk M, Stigloher C, Steger M, Schlosser A, Ohlsen K (2018)
The serine/threonine kinase Stk and the phosphatase Stp regulate cell wall synthesis in Staphylococcus aureus
Scientific Reports 12;8(1):13693

Bruchhagen C, Jarick M, Mewis C, Hertlein T, Niemann S, Ohlsen K, Peters G, Planz O, Ludwig S, Ehrhardt C (2018)
Metabolic conversion of CI-1040 turns a cellular MEK-inhibitor into an antibacterial compound
Scientific Reports 14;8(1):9114

Ohlsen K, Hertlein T (2018)
Towards clinical application of non-invasive imaging to detect bacterial infections

Virulence
9(1):943-945

El-Hossary EM, Förstner KU, François P, Baud D, Streker K, Schrenzel J, Ohlsen K, Holzgrabe U (2018)
A Novel Mechanism of Inactivating Antibacterial Nitro Compounds
in the Human Pathogen Staphylococcus aureus by Overexpression of a NADH-Dependent Flavin Nitroreductase
Antimicrob Agents Chemother 25;62(2) e01510-17

Ashraf K, Yasrebi K, Hertlein T, Ohlsen K, Lalk M, Hilgeroth A (2017)
Novel Effective Small-Molecule Antibacterials against Enterococcus Strains
Molecules 10;22(12). pii: E2193. doi: 10.3390

Mielich-Süss B, Wagner RM, Mietrach N, Hertlein T, Marincola G, Ohlsen K, Geibel S, Lopez D (2017)
Flotillin scaffold activity contributes to type VII secretion system assembly in Staphylococcus aureus
PLoS Pathog 22;13(11):e1006728. doi: 10.1371

García-Betancur JC, Goñi-Moreno A, Horger T, Schott M, Sharan M, Eikmeier J, Wohlmuth B, Zernecke A, Ohlsen K, Kuttler C, Lopez D (2017)
Cell differentiation defines acute and chronic infection cell types in Staphylococcus aureus
Elife 12;6. pii: e28023. doi: 10.7554

Popp C, Hampe IAI, Hertlein T, Ohlsen K, Rogers PD, Morschhäuser J (2017)
Competitive fitness of fluconazole-resistant clinical Candida albicans strains
Antimicrob Agents Chemother 27;61(7)

Ramírez-Zavala B, Mottola A, Haubenreißer J, Schneider S, Allert S, Brunke S, Ohlsen K, Hube B, Morschhäuser J (2017)
The Snf1-activating kinase Sak1 is a key regulator of metabolic adaptation and in vivo fitness of Candida albicans
Mol Microbiol 104:989-1007

Nguyen MT, Luqman A, Bitschar K, Hertlein T, Dick J, Ohlsen K, Bröker B, Schittek B, Götz F (2017)
Staphylococcal (phospho)lipases promote biofilm formation and host cell invasion
Int J Med Microbiol  pii: S1438-4221(17)30312-0.doi: 10.1016

Hardt P, Engels I, Rausch M, Gajdiss M, Ulm H, Sass P, Ohlsen K, Sahl HG, Bierbaum G, Schneider T, Grein F (2017)
The cell wall precursor lipid II acts as a molecular signal for the Ser/Thr kinase PknB of Staphylococcus aureus
International Journal of Medical Microbiology 307(1):1-10

El-Hossary EM, Cheng C, Hamed MM, El-Sayed Hamed AN, Ohlsen K, Hentschel U, Abdelmohsen UR (2017)
Antifungal potential of marine natural products
European Journal of Medicinal Chemistry 126:631-651

Blättner S, Das S, Paprotka K, Eilers U, Krischke M, Kretschmer D, Remmele CW, Dittrich M, Müller T, Schuelein-Voelk C, Hertlein T, Mueller MJ, Huettel B, Reinhardt R, Ohlsen K, Rudel T, Fraunholz M
Staphylococcus aureus Exploits a Non-ribosomal Cyclic Dipeptide to Modulate Survival within Epithelial Cells and Phagocytes
PLoS Pathogen 15;12:e1005857

Das S, Lindemann C, Young BC, Muller J, Österreich B, Ternette N, Winkler AC, Paprotka K, , Reinhardt R, Förstner KU, Allen E, Flaxman A, Yamaguchi Y, Rollier CS, van Diemen P, Blättner S, Remmele CW, Selle M, Dittrich M, Mueller T, Vogel J, Ohlsen K, Crook DW, Massey R, Wilson DJ, Rudel R, Wyllie DH, Fraunholz MJ (2016)
Natural mutations in a Staphylococcus aureus virulence regulator attenuate cytotoxicity but permit bacteremia and abscess formation
PNAS 31;113:E3101-10

Selle M, Hertlein T, Oesterreich B, Klemm T, Kloppot P, Müller E, Ehricht R, Stentzel S, Bröker BM, Engelmann S, Ohlsen K (2016)
Global antibody response to Staphylococcus aureus live-cell vaccination.
Science Reports 6:24754

El-Sayed MT, Zoraghi R, Reiner N, Suzen S, Ohlsen K, Lalk M, Altanlar N, Hilgeroth A (2015)
Novel inhibitors of the methicillin-resistant Staphylococcus aureus (MRSA)-pyruvate kinase.
J Enzyme Inhib Med Chem 10:1-6

Kutscher M, Rosenberger M, Schmauss B, Meinel L, Lorenz U, Ohlsen K, Hellmann R, Germershaus O (2015)
Surface functionalization allowing repetitive use of optical sensors for real-time detection of antibody-bacteria interaction
J Biophotonics Oct 21. doi: 10.1002/jbio.201500178

Nguyen MT, Kraft B, Yu W, Demicrioglu DD, Hertlein T, Burian M, Schmaler M, Boller K, Bekeredjian-Ding I, Ohlsen K, Schittek B, Götz F (2015)
The
?Sa? Specific Lipoprotein Like Cluster (lpl) of S. aureus USA300 Contributes to Immune Stimulation and Invasion in Human Cells
PLoS Pathogens 11:e1004984

Cecil A, Ohlsen K, Menzel T, François P, Schrenzel J, Fischer A, Dörries K, Selle M, Lalk M, Hantzschmann J, Dittrich M, Liang C, Bernhardt J, Olschläger TA, Bringmann G, Bruhn H, Unger M, Ponte-Sucre A, Lehmann L, Dandekar T (2015)
Modelling antibiotic and cytotoxic isoquinoline effects in Staphylococcus aureus, Staphylococcus epidermidis and mammalian cells
International Journal of Medical Microbiology 305:96-109

Koch G, Yepes A, Förstner KU, Wermser C, Stengel S, Modamio S, Ohlsen K, Foster K, Lopez D (2014)
Evolution of resistance to a last-resort antibiotic in Staphyloccocus aureus via bacterial competition
Cell 158, 1060–1071

Grosz M, Kolter J, Paprotka K, Winkler AC, Schäfer D, Chatterjee SS, Geiger T, Wolz C, Ohlsen K, Otto M, Rudel T, Sinha B, Fraunholz M (2014)
Cytoplasmic replication of Staphylococcus aureus upon phagosomal escape triggered by phenol-soluble modulin alpha
Cell Microbiol 16(4):451-65

Bäsell K, Otto A, Junker S, Zühlke D, Rappen GM, Schmidt S, Hentschker C, Macek B, Ohlsen K, Hecker M, Becher D (2013)
The phosphoproteome and its physiological dynamics in Staphylococcus aureus
Int J Med Microbiol S1438-4221(13)193-8

Yu W, Leibig M, Schäfer T, Bertram R, Ohlsen K, Götz F (2013)
Mevalonate auxotrophic mutant of Staphylococcus aureus can adapt to mevalonate depletion
Antimicrob Agents Chemother Aug 19. [Epub ahead of print]

Hertlein, T, Sturm V, Jakob P, Ohlsen K (2013)
19F Magnetic Resonance Imaging of perfluorocarbons for the evaluation of response to antibiotic therapy in a Staphylococcus aureus infection model
PLoS One 8(5):e64440

Dunkel N, Hertlein T, Franz R, Reuß O, Sasse C, Schäfer T, Ohlsen K, Morschhäuser J
Roles of different peptide transporters in nutrient acquisition in Candida albicans
Eukaryot Cell 2013 Apr;12(4):520-8

Feng J, Michalik S, Varming AN, Andersen JH, Albrecht D, Jelsbak L, Krieger S, Ohlsen K, Hecker M, Gerth U, Ingmer H, Frees D (2013)
Trapping and Proteomic Identification of Cellular Substrates of the ClpP Protease in Staphylococcus aureus
J Proteome Res in press

Sasse C, Dunkel N, Schäfer T, Schneider S, Dierolf F, Ohlsen K, Morschhäuser J (2012)
The stepwise acquisition of fluconazole resistance mutations causes a gradual loss of fitness in Candida albicans
Mol Microbiol 86(3):539-56

Marincola G, Schäfer T, Behler J, Bernhardt J, Ohlsen K, Goerke C, Wolz C (2012)
RNase Y of Staphylococcus aureus and its role in the activation of virulence genes
Mol Microbiol 85:817-832

Rakette S, Donat S, Ohlsen K, Stehle T (2012)
Structural Analysis of Staphylococcus aureus Serine/Threonine Kinase PknB
PLoS One 7(6):e39136

Tischer M, Pradel G, Ohlsen K, Holzgrabe U (2012)
Quaternary ammonium salts and their antimicrobial potential: targets or nonspecific interactions?
ChemMedChem 2;7(1):22-31

Depke M, Burian M, Schäfer T, Bröker BM, Ohlsen K, Völker U (2012)
The alternative sigma factor B modulates virulence gene expression in a murine Staphylococcus aureus infection model but does not influence kidney gene expression pattern of the host
Int J Med Microbiol 302(1):33-9

Donat S, Hasenberg M, Schäfer T, Ohlsen K, Gunzer M, Einsele H, Löffler J, Beilhack A, Krappmann S (2012)
Surface display of Gaussia princeps luciferase allows sensitive fungal pathogen detection during cutaneous aspergillosis
Virulence 3(1) [Epub ahead of print]

Hoffmann C, Ohlsen K, Hauck CR (2011)
Integrin-mediated uptake of fibronectin-binding bacteria
Eur J Cell Biol 2011 May 9 [Epub ahead of print]

Kusch K, Hanke K, Holtfreter S, Schmudde M, Kohler C, Erck C, Wehland J, Hecker M, Ohlsen K, Bröker B, Engelmann S (2011)
The influence of SaeRS and σ(B) on the expression of superantigens in different Staphylococcus aureus isolates
Int J Med Microbiol 301(6):488-99

Hertlein T, Sturm V, Kircher S, Basse-Lüsebrink T, Haddad D, Ohlsen K, Jakob P (2011)
Visualization of abscess formation in a murine thigh infection model of Staphylococcus aureus by 19F-magnetic resonance imaging (MRI)
PLoS One 24;6(3):e18246

Cecil A, Rikanović C, Ohlsen K, Liang C, Bernhardt J, Oelschlaeger TA, Gulder T, Bringmann G, Holzgrabe U, Unger M, Dandekar T (2011)
Modeling antibiotic and cytotoxic effects of the dimeric isoquinoline IQ-143 on metabolism and its regulation in Staphylococcus aureus, Staphylococcus epidermidis and human cells
Genome Biol 21;12(3):R24

Lorenz U, Lorenz B, Schmitter T, Streker K, Erck C, Wehland J, Nickel J,Zimmermann B, Ohlsen K (2011)
Functional antibodies targeting IsaA of Staphylococcus aureus augment host immune response and open new perspectives for antibacterial therapy
Antimicrob Agents Chemother 55(1):165-73

Menzel TM, Tischer M, François P, Nickel J, Schrenzel J, Bruhn H, Albrecht A, Lehmann L, Holzgrabe U, Ohlsen K (2010)
Mode-of-action studies of the novel bisquaternary bisnaphthalimide MT02 against Staphylococcus aureus
Antimicrob Agents Chemother 55(1):311-20

Liebeke M, Meyer H, Donat S, Ohlsen K, Lalk M (2010)
A metabolomic view of Staphylococcus aureus and its ser/thr kinase and phosphatase deletion mutants: involvement in cell wall biosynthesis
Chem Biol 27;17(8):820-30

Ohlsen K, Lorenz U (2010)
Immunotherapeutic strategies to combat staphylococcal Infections
Int J Med Microbiol 300(6):402-10

Sibbald MJ, Winter T, van der Kooi-Pol MM, Buist G, Tsompanidou E, Bosma T, Schäfer T, Ohlsen K, Hecker M, Antelmann H, Engelmann S, van Dijl JM (2010)
Synthetic effects of secG and secY2 mutations on exoproteome biogenesis in Staphylococcus aureus J Bacteriol 192(14):3788-800

Kozytska S, Stauss D, Pawlik MC, Hensen S, Eckart M, Ziebuhr W, Witte W,Ohlsen K (2010)
Identification of specific genes in Staphylococcus aureus strains associated with bovine mastitis
Vet Microbiol 26;145(3-4):360-5

Hoffmann C, Berking A, Agerer F, Buntru A, Neske F, Chhatwal GS, Ohlsen K,Hauck CR (2010)
Caveolin limits membrane microdomain mobility and integrin-mediated uptake of fibronectin-binding pathogens
J Cell Sci 15;123 (Pt24):4280-91

Herbert S, Ziebandt AK, Ohlsen K, Schäfer T, Hecker M, Albrecht D, Novick R, Götz F (2010)
Repair of global regulators in Staphylococcus aureus 8325 and comparative analysis with other clinical isolates
Infect Immun 78(6):2877-89

Geiger T, Goerke C, Fritz M, Schäfer T, Ohlsen K, Liebeke M, Lalk M, Wolz C (2010)
Role of the (p)ppGpp synthase RSH, a RelA/SpoT homolog, in stringent response and virulence of Staphylococcus aureus
Infect Immun 78(5):1873-83

Miller M, Donat S, Rakette S, Stehle T, Kouwen TR, Diks SH, Dreisbach A, Reilman E, Gronau K, Becher D, Peppelenbosch MP, van Dijl JM, Ohlsen K (2010)
Staphylococcal PknB as the first prokaryotic representative of the proline-directed kinases
PLoS One 4;5(2):e9057

Cuny C, Friedrich A, Kozytska S, Layer F, Nübel U, Ohlsen K, Strommenger B, Walther B, Wieler L, Witte W (2010)
Emergence of methicillin-resistant Staphylococcus aureus (MRSA) in different animal species
Int J Med Microbiol 300(2-3):109-17

Chlebowicz MA, Nganou K, Kozytska S, Arends JP, Engelmann S, Grundmann H, Ohlsen K, van Dijl JM, Buist G (2010)
Recombination between ccrC genes in a type V (5C2&5) staphylococcal cassette chromosome mec (SCCmec) of Staphylococcus aureus  ST398 leads to conversion from methicillin resistance to methicillin susceptibility in vivo
Antimicrob Agents Chemother 54(2):783-91

Ohlsen K, Donat S. (2010)
The impact of serine/threonine phosphorylation in Staphylococcus aureus
Int J Med Microbiol 300(2-3):137-41

Jelsbak L, Hemmingsen L, Donat S, Ohlsen K, Boye K, Westh H, Ingmer H, Frees D (2010)
Growth phase-dependent regulation of the global virulence regulator Rot in clinical isolates of Staphylococcus aureus
Int J Med Microbiol 300(4):229-36

Biswas L, Biswas R, Nerz C, Ohlsen K, Schlag M, Schäfer T, Lamkemeyer T, Ziebandt AK, Hantke K, Rosenstein R, Götz F (2009)
Role of the twin-arginine translocation pathway in Staphylococcus
J Bacteriol 191(19):5921-9

Donat S, Streker K, Schirmeister T, Rakette S, Stehle T, Liebeke M, Lalk M,Ohlsen K (2009)
Transcriptome and functional analysis of the eukaryotic-type serine/threonine kinase PknB in Staphylococcus aureus
J Bacteriol 191(13):4056-69

Ohlsen K, Dandekar G, Schwarz R, Dandekar T (2008)
New trends in pharmacogenomic strategies against resistance development in microbial infections
Pharmacogenomics 9(11):1711-23. Review

Streker K, Schäfer T, Freiberg C, Brötz-Oesterhelt H, Hacker J, Labischinski H, Ohlsen K
In vitro and in vivo validation of ligA and tarI as essential targets in Staphylococcus aureus
Antimicrob Agents Chemother 52(12):4470-4

Ramirez-Zavala B, Reuss O, Park YN, Ohlsen K, Morschhäuser J. (2008)
Environmental induction of white-opaque switching in Candida albicans
PLoS Pathog
13;4(6):e1000089

Lorenz U, Hüttinger C, Schäfer T, Ziebuhr W, Thiede A, Hacker J, Engelmann S, Hecker M, Ohlsen K (2008)
The alternative sigma factor sigma B of Staphylococcus aureus modulates virulence in experimental central venous catheter-related infections
Microbes Infect. 10(3):217-23. Epub 2007 Nov 28. PubMed PMID: 18328762.

Ohlsen K, Lorenz U (2007)
Novel targets for antibiotics in Staphylococcus aureus
Future Microbiol 2(6):655-66

Besier S, Ludwig A, Ohlsen K, Brade V, Wichelhaus TA (2007)
Molecular analysis of the thymidine-auxotrophic small colony variant phenotype of Staphylococcus aureus
Int J Med Microbiol 297(4):217-25.

Michel A, Agerer F, Hauck CR, Herrmann M, Ullrich J, Hacker J, Ohlsen K (2006)
Global regulatory impact of ClpP protease of Staphylococcus aureus on regulons involved in virulence, oxidative stress response, autolysis, and DNA repair
J Bacteriol 188(16):5783-96

Hauck CR, Ohlsen K. (2006)
Sticky connections: extracellular matrix protein recognition and integrin-mediated cellular invasion by Staphylococcus aureus
Curr Opin Microbiol 9(1):5-11

Kozitskaya S, Olson ME, Fey PD, Witte W, Ohlsen K, Ziebuhr W. (2005)
Clonal analysis of Staphylococcus epidermidis isolates carrying or lacking biofilm-mediating genes by multilocus sequence typing J Clin Microbiol. 43(9):4751-7.

Harraghy N, Kormanec J, Wolz C, Homerova D, Goerke C, Ohlsen K, Qazi S, HillP, Herrmann M (2005)
sae is essential for expression of the staphylococcal adhesins Eap and Emp
Microbiology 151(Pt 6):1789-800.

Agerer F, Lux S, Michel A, Rohde M, Ohlsen K, Hauck CR (2005)
Cellular invasion byStaphylococcus aureus reveals a functional link between focal adhesion kinase and cortactin in integrin-mediated internalisation J Cell Sci. 15;118(Pt 10):2189-200

Ohlsen K, Hacker J. (2005)
Infections in the elderly
Int J Med Microbiol 294(8):471-2

Streker K, Freiberg C, Labischinski H, Hacker J, Ohlsen K.
Staphylococcus aureus NfrA (SA0367) is a flavin mononucleotide-dependent NADPH oxidase involved in oxidative stress response
J Bacteriol 2005 Apr;187(7):2249-56

Ziebandt AK, Becher D, Ohlsen K, Hacker J, Hecker M, Engelmann S (2004)
The influence of agr and sigmaB in growth phase dependent regulation of virulence factors in Staphylococcus aureus
Proteomics 4(10):3034-47.

Agerer F, Michel A, Ohlsen K, Hauck CR (20039
Integrin-mediated invasion of Staphylococcus aureus into human cells requires Src family protein-tyrosine kinases
J Biol Chem 24;278(43):42524-31

Ohlsen K, Ternes T, Werner G, Wallner U, Löffler D, Ziebuhr W, Witte W, Hacker J (2003)
Impact of antibiotics on conjugational resistance gene transfer in Staphylococcus aureus in sewage
Environ Microbiol 5(8):711-6